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Chemotherapy in Haematology

📋 Key Information Summary

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  • Haematological malignancies — leukaemias, lymphomas, and myeloma — are among the most chemotherapy-sensitive cancers, with many regimens offering curative or long-term disease control.
  • CHOP (cyclophosphamide, doxorubicin, vincristine, prednisolone) ± rituximab (R-CHOP) is the standard first-line regimen for aggressive B-cell non-Hodgkin lymphoma (NHL).
  • ABVD (doxorubicin, bleomycin, vinblastine, dacarbazine) remains first-line for Hodgkin lymphoma; bleomycin pulmonary toxicity requires baseline and serial pulmonary function monitoring.
  • 7+3 induction (cytarabine 7 days + anthracycline 3 days) is the backbone for acute myeloid leukaemia (AML) intensive remission induction; azacitidine-based regimens are standard for unfit patients.
  • FCR (fludarabine, cyclophosphamide, rituximab) is effective for fit, younger patients with chronic lymphocytic leukaemia (CLL), though venetoclax-based therapies are increasingly first-line.
  • Anthracyclines (doxorubicin, idarubicin) carry cumulative cardiotoxicity risk; echocardiography is mandatory before treatment and at cumulative doses ≥300 mg/m² doxorubicin equivalent.
  • Alkylating agents (cyclophosphamide, bendamustine, busulfan) cause myelosuppression, gonadal toxicity, and secondary malignancy risk; fertility preservation must be discussed pre-treatment.
  • Tumour lysis syndrome (TLS) prophylaxis with rasburicase and aggressive hydration is critical for high-burden lymphomas and AML induction — assess risk with the Cairo–Bishop criteria.
  • Neutropenic fever (febrile neutropenia) is a medical emergency; empirical IV antibiotics (piperacillin-tazobactam) must be administered within 60 minutes of presentation per ASCQHC standards.
  • Aboriginal and Torres Strait Islander peoples have higher incidence and mortality from lymphoma and leukaemia with later diagnosis; culturally safe, community-based supportive care improves outcomes.
  • All chemotherapy regimens must be prescribed using electronic prescribing with double-check verification; oral chemotherapy requires documented patient education and adherence monitoring.
  • Pregnancy and fertility considerations are paramount — alkylating agents are teratogenic; referral for sperm or oocyte cryopreservation should occur before the first cycle.

Introduction & Australian Epidemiology

Haematological malignancies encompass a diverse group of cancers arising from blood, bone marrow, lymph nodes, and lymphoid tissues. In Australia, approximately 17,000 new cases of blood cancer are diagnosed annually, making haematological malignancies the third most common cancer group and the second leading cause of cancer-related death. Lymphoma, leukaemia, and myeloma collectively account for over 6,500 deaths per year.

Treatment with systemic chemotherapy remains the cornerstone of management for most haematological malignancies. Unlike many solid organ cancers, haematological malignancies are often highly chemosensitive, and cure or durable remission is achievable with appropriate multi-agent regimens. Specific combinations — such as CHOP for aggressive lymphoma, ABVD for Hodgkin lymphoma, 7+3 for acute myeloid leukaemia, and FCR for chronic lymphocytic leukaemia — have been refined over decades and remain central to Australian treatment protocols.

The Australian burden of haematological malignancies is notable for several features: a rising incidence of diffuse large B-cell lymphoma in older adults, a significant gap in outcomes for Aboriginal and Torres Strait Islander peoples, and increasing adoption of targeted therapies and immunotherapies that are supplementing — though not yet replacing — cytotoxic chemotherapy. All chemotherapy must be delivered within accredited facilities meeting NSQHS Standards and under the supervision of a credentialed haematologist or medical oncologist.

Chemotherapy in Haematology clinical infographic — pathophysiology, clinical clues, diagnosis, imaging, and management
Tap or click image to enlarge — Chemotherapy in Haematology: pathophysiology, clinical clues, diagnosis, imaging, and management.
Chemotherapy in Haematology infographic, full size

Principles of Haematological Chemotherapy

Haematological chemotherapy is guided by several foundational principles that distinguish it from solid tumour oncology:

  • Curative intent: Many regimens (e.g. ABVD for Hodgkin lymphoma, 7+3 for AML) are administered with curative intent, requiring optimal dose intensity and adherence to schedule.
  • Combination therapy: Multi-agent regimens exploit non-overlapping toxicities and different mechanisms of action to overcome resistance. Single-agent chemotherapy is rarely appropriate for first-line haematological malignancies.
  • Cycle-based dosing: Regimens are delivered in defined cycles (typically 21- or 28-day intervals), allowing bone marrow recovery between administrations.
  • Dose modification protocols: Standardised dose-reduction and escalation criteria exist for renal impairment (e.g. cytarabine), hepatic dysfunction, and haematological toxicity.
  • Response assessment: Interim and end-of-treatment imaging (PET-CT for lymphoma) and bone marrow biopsy (for leukaemia/myeloma) guide decisions regarding treatment continuation, consolidation, or intensification.
  • Supportive care integration: Antiemetics, growth factor support (G-CSF), tumour lysis prophylaxis, infection prophylaxis, and psychosocial support are integral components of every regimen.
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Medication safety: All haematological chemotherapy must be prescribed via electronic prescribing systems with independent double-check verification at dispensing, preparation, and administration. Verbal orders are not acceptable for chemotherapy. Blood product irradiation is required for patients receiving purine analogue therapy (fludarabine, cladribine) due to risk of transfusion-associated graft-versus-host disease.

Phases of Haematological Chemotherapy

Phase Purpose Examples
Induction Achieve complete remission / maximum tumour reduction 7+3 (AML), CHOP ×6 (NHL), VTD (myeloma)
Consolidation Eradicate residual disease; reduce relapse risk High-dose cytarabine (AML), autologous SCT (lymphoma, myeloma)
Maintenance Prolong remission in incurable or high-risk disease Lenalidomide (myeloma), rituximab (FL), azacitidine (MDS)
Salvage Re-induction after relapse or refractory disease R-ICE, R-DHAP (lymphoma), FLAG-Ida (AML)

Key Drug Mechanisms

Understanding the mechanisms of action of core cytotoxic agents is essential for anticipating toxicities, managing drug interactions, and selecting appropriate regimens.

Anthracyclines

Anthracyclines are among the most active agents in haematological oncology. They intercalate into DNA, inhibit topoisomerase II, generate free radicals, and cause direct DNA strand breaks. They are essential components of CHOP, ABVD, and 7+3 regimens.

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Doxorubicin
Adriamycin® · Anthracycline antibiotic
Adult dose 50 mg/m² IV on Day 1 (CHOP); 25 mg/m² IV on Days 1 & 15 (ABVD)
Paediatric dose Dose per protocol; typically 30–60 mg/m² IV per cycle
Route IV — central line preferred due to vesicant risk
Key toxicity Cardiomyopathy (cumulative dose >450 mg/m²); acute cardiotoxicity; myelosuppression; alopecia
Renal adjustment No adjustment required
PBS status ✔ PBS General Benefit
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Idarubicin
Zavedos® · Anthracycline antibiotic
Adult dose 12 mg/m² IV daily ×3 days (Days 1–3 in 7+3 induction)
Route IV — slow push over 10–15 minutes via central line
Key toxicity Cumulative cardiotoxicity (lower threshold ~150 mg/m² due to higher potency); severe myelosuppression
PBS status ✔ PBS General Benefit
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Daunorubicin
Cerubidine® · Anthracycline antibiotic
Adult dose 60 mg/m² IV daily ×3 days (7+3 induction, per Australian AML protocols)
Key toxicity Cardiomyopathy; mucositis; myelosuppression
PBS status ✔ PBS General Benefit
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Cardiotoxicity monitoring: Echocardiography or MUGA scan is mandatory before initiating anthracycline therapy and should be repeated before each cycle if clinical concern arises. Patients who have received cumulative doxorubicin ≥300 mg/m² or idarubicin equivalent require ongoing cardiac surveillance. Dexrazoxane may be considered for cardiac protection in patients requiring continued anthracycline exposure beyond cumulative thresholds.

Alkylating Agents

Alkylating agents form covalent bonds with DNA, causing cross-links that prevent replication. They are active across the cell cycle (non-phase-specific) and form the backbone of many haematological regimens.

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Cyclophosphamide
Endoxan® · Nitrogen mustard alkylator
Adult dose 750 mg/m² IV Day 1 (CHOP); 500 mg/m² IV Days 1 & 3 (FCR)
Paediatric dose Protocol-dependent; 300–1200 mg/m² IV per dose
Route IV (standard); PO for maintenance (50–100 mg daily)
Key toxicity Haemorrhagic cystitis (prevent with mesna if high-dose); myelosuppression; gonadal toxicity; secondary malignancy
Renal adjustment Dose reduction of 25% if CrCl <10 mL/min
PBS status ✔ PBS General Benefit
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Bendamustine
Ribomustin® · Bifunctional alkylator
Adult dose 90 mg/m² IV Days 1 & 2 (BR regimen for CLL/NHL); 100 mg/m² IV Days 1 & 2 (CLL)
Key toxicity Myelosuppression; tumour lysis; infusion reactions; secondary malignancy (skin cancer surveillance required)
PBS status ⚠ PBS Restricted Benefit

Antimetabolites

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Cytarabine (Ara-C)
Cytosar® · Pyrimidine antimetabolite
Adult dose (7+3 induction) 100 mg/m²/day CIVI for 7 days
High-dose consolidation 3 g/m² IV over 3 hours q12h ×6 doses (Days 1, 3, 5)
Key toxicity Cerebellar toxicity (dose-limiting, especially >60 yrs); keratitis/conjunctivitis (use prophylactic dexamethasone eye drops); myelosuppression
Renal adjustment No adjustment for standard dose; consider dose reduction for HD-Ara-C if renal impairment
PBS status ✔ PBS General Benefit
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Fludarabine
Fludara® · Purine antimetabolite
Adult dose 25 mg/m² IV daily ×3 days (Days 1–3 in FCR)
Key toxicity Severe immunosuppression (CD4+ depletion lasting 6–12 months); neurotoxicity at high doses; transfusion-associated GVHD (irradiate all blood products)
Renal adjustment Dose reduce by 50% if CrCl 30–70 mL/min; contraindicated if CrCl <30 mL/min
PBS status ✔ PBS General Benefit

Chemotherapy Regimens

The following regimens represent the most commonly used chemotherapy protocols in Australian haematology practice. All doses and schedules should be verified against institutional protocols and current PBS listings.

CHOP / R-CHOP — Aggressive B-Cell Non-Hodgkin Lymphoma

R-CHOP-21 (rituximab, cyclophosphamide, doxorubicin, vincristine, prednisolone) administered every 21 days for 6–8 cycles is the standard first-line treatment for diffuse large B-cell lymphoma (DLBCL) and other aggressive B-cell NHL.

Drug Dose Route Day(s)
Rituximab 375 mg/m² IV infusion 1
Cyclophosphamide 750 mg/m² IV 1
Doxorubicin 50 mg/m² IV 1
Vincristine 1.4 mg/m² (max 2 mg) IV 1
Prednisolone 100 mg PO 1–5
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PBS status: Rituximab (MabThera®) is PBS-listed for DLBCL and follicular lymphoma under Section 100 (Specialist/Authority Required). Biosimilars (Riximyo®, Truxima®) are available and PBS-interchangeable.

ABVD — Hodgkin Lymphoma

ABVD (doxorubicin, bleomycin, vinblastine, dacarbazine) administered every 28 days for 2–6 cycles (with interim PET-adapted de-escalation per RATHL study) is first-line for classical Hodgkin lymphoma.

Drug Dose Route Day(s)
Doxorubicin 25 mg/m² IV 1 & 15
Bleomycin 10 units/m² IV 1 & 15
Vinblastine 6 mg/m² IV 1 & 15
Dacarbazine 375 mg/m² IV 1 & 15
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Bleomycin pulmonary toxicity: Monitor DLCO and spirometry at baseline and after every 2 cycles. Discontinue bleomycin if DLCO declines >25% from baseline. Cumulative dose should not exceed 270 units/m². Avoid supplemental oxygen during surgery in patients with prior bleomycin exposure.

7+3 — Acute Myeloid Leukaemia (AML) Induction

The 7+3 regimen is the standard intensive remission induction for AML in patients fit enough for intensive chemotherapy (typically <70 years with good performance status).

Drug Dose Route Duration
Cytarabine 100 mg/m²/day continuous IV infusion CIVI 7 days (Days 1–7)
Idarubicin 12 mg/m²/day IV IV push 3 days (Days 1–3)

Alternatives for unfit/elderly patients: Azacitidine (75 mg/m² SC/IV daily ×7 days per 28-day cycle — PBS Authority Required) ± venetoclax; low-dose cytarabine; or best supportive care with hydroxyurea for cytoreduction.

FCR — Chronic Lymphocytic Leukaemia (CLL)

FCR (fludarabine, cyclophosphamide, rituximab) is an intensive regimen for fit patients with CLL, particularly those with mutated IGHV and del(13q) who may achieve very long remissions. Increasingly, venetoclax-based combinations (VenR, VenO) are preferred as first-line due to superior tolerability and efficacy.

Drug Dose Route Day(s)
Fludarabine 25 mg/m² IV 1–3
Cyclophosphamide 250 mg/m² IV 1–3
Rituximab 375 mg/m² (Cycle 1); 500 mg/m² (Cycles 2–6) IV 1

Repeat every 28 days for 6 cycles. All blood products must be irradiated. Pneumocystis jirovecii prophylaxis (trimethoprim-sulfamethoxazole DS three times weekly) and antiviral prophylaxis (valaciclovir 500 mg daily) are mandatory.

Regimen Comparison Overview

Regimen Indication Cycles Intent Key Risk
R-CHOP DLBCL, aggressive B-NHL 6–8 × 21 days Curative Cardiotoxicity, neuropathy
ABVD Classical Hodgkin lymphoma 2–6 × 28 days Curative Pulmonary fibrosis (bleomycin)
7+3 AML induction 1 (+ consolidation) Curative Prolonged aplasia, infection
FCR CLL (fit patients) 6 × 28 days Long-term control Immunosuppression, secondary MDS/AML

Toxicity Management & Supportive Care

Effective management of chemotherapy toxicities is essential for maintaining dose intensity, preventing life-threatening complications, and preserving quality of life.

Febrile Neutropenia

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Time-critical emergency: Febrile neutropenia (temperature ≥38.0°C with ANC <0.5 × 10⁹/L) requires empirical broad-spectrum IV antibiotics within 60 minutes. Use the MASCC score to stratify risk.
Low Risk
MASCC Score ≥21
Stable haemodynamics, no comorbidities, outpatient consideration.
Setting: Consider oral antibiotics (amoxicillin-clavulanate + ciprofloxacin) if outpatient pathway
High Risk
MASCC Score <21
Haemodynamic instability, comorbidities, anticipated >7 days neutropenia.
Setting: Hospital admission — IV piperacillin-tazobactam 4.5 g q8h (1st line)
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Piperacillin-Tazobactam
Tazocin® · β-lactam/β-lactamase inhibitor
Adult dose 4.5 g IV q8h (extended infusion over 3 hours preferred)
Paediatric dose 100 mg/kg (of piperacillin component) IV q8h
Renal adjustment Dose reduce if CrCl <40 mL/min
PBS status ✔ PBS General Benefit

Tumour Lysis Syndrome (TLS) Prophylaxis

TLS risk assessment (Cairo–Bishop criteria) must be performed before commencing chemotherapy for high-burden haematological malignancies. TLS is most common in AML induction, high-grade lymphomas (especially Burkitt), and ALL.

Low Risk
<1% TLS incidence
Low tumour burden, solid tumours.
Oral hydration + allopurinol 300 mg daily
Intermediate Risk
1–5% TLS incidence
DLBCL, ALL (without very high WCC).
IV hydration 2–3 L/day + allopurinol; consider rasburicase if uric acid rising
High Risk
>5% TLS incidence
Burkitt lymphoma, AML with WCC >100 × 10⁹/L, high-grade transformation.
IV hydration 3–4 L/day + rasburicase 0.2 mg/kg IV (single dose, repeat PRN); ICU consideration
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Rasburicase
Fasturtec® · Recombinant urate oxidase
Adult dose 0.2 mg/kg IV over 30 min; may repeat at 24h if uric acid still elevated
Key precaution Contraindicated in G6PD deficiency — risk of severe haemolysis. Screen with G6PD assay before first dose.
PBS status ⚠ PBS Authority Required

Antiemetic Protocols

Haematological regimens vary in emetogenic risk. R-CHOP and 7+3 are moderately to highly emetogenic; ABVD is moderately emetogenic.

Emetogenic Risk Antiemetic Regimen Duration
High (7+3, HyperCVAD) Aprepitant 125 mg PO Day 1 + 80 mg Days 2–3; dexamethasone 12 mg PO/IV Days 1–4; ondansetron 8 mg IV/PO BD Days 1–4 Days 1–4 per cycle
Moderate (R-CHOP, ABVD) Ondansetron 8 mg IV/PO BD Day 1; dexamethasone 8 mg PO Days 1–3 (given with prednisolone in CHOP) Days 1–3
Breakthrough Levomepromazine 6.25 mg PO/SC PRN; lorazepam 0.5–1 mg SL for anticipatory nausea As needed

Growth Factor Support (G-CSF)

Granulocyte colony-stimulating factor (G-CSF) is used for primary prophylaxis when the anticipated risk of febrile neutropenia is ≥20%, or for secondary prophylaxis after a prior febrile neutropenia episode.

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Filgrastim
Neupogen® / Zarzio® (biosimilar) · G-CSF
Adult dose 5 mcg/kg SC daily; start 24–72h post-chemotherapy; continue until ANC >1.0 × 10⁹/L
PBS status ⚠ PBS Authority Required
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Pegfilgrastim
Neulasta® / Ziextenzo® (biosimilar) · Long-acting G-CSF
Adult dose 6 mg SC once; administer ≥24h post-chemotherapy
PBS status ⚠ PBS Authority Required

Other Supportive Care Measures

  • Infection prophylaxis: Trimethoprim-sulfamethoxazole (PO 480 mg BD three times weekly) for Pneumocystis prophylaxis in patients receiving purine analogues, high-dose corticosteroids, or prolonged neutropenia. Valaciclovir 500 mg daily for HSV/VZV prophylaxis with fludarabine or alemtuzumab.
  • Fertility preservation: Counsel all patients of reproductive age before commencing alkylating agents. Refer to a fertility specialist for sperm cryopreservation (males) or oocyte/embryo cryopreservation (females). Ganirelix/ GnRH agonist co-administration may reduce gonadotoxicity in females.
  • Mucositis management: Maintain oral hygiene with chlorhexidine 0.2% mouthwash. Morphine patient-controlled analgesia for severe mucositis. Cryotherapy during short-infusion melphalan reduces mucositis severity.
  • Venous thromboembolism (VTE) prophylaxis: Thalidomide and lenalidomide carry high VTE risk — mandatory thromboprophylaxis with aspirin (low risk) or LMWH (high risk) per IMWG guidelines.

Special Populations

🤰 Pregnancy
Alkylating agents (cyclophosphamide)
Teratogenic in first trimester (Category D). May be used in 2nd/3rd trimester if clinically necessary under specialist guidance.
Methotrexate
Absolutely contraindicated in pregnancy (Category X) — potent folate antagonist, causes neural tube defects.
Doxorubicin
Limited safety data; avoid in first trimester if possible. Anthracycline-based therapy may be used in 2nd/3rd trimester for aggressive lymphoma when life-threatening.
Rituximab
Crosses placenta in 2nd/3rd trimester; may cause neonatal B-cell depletion. Avoid if possible; risk-benefit discussion essential.
👶 Paediatrics
Vincristine
Standard in ALL protocols; dose by BSA. Capped at 2 mg in adults; no cap in paediatrics. Neurotoxicity (constipation, jaw pain) more prominent in children.
L-asparaginase
Cornerstone of paediatric ALL therapy; monitor for hypersensitivity, pancreatitis, and thrombosis. Native E. coli or pegylated formulations available.
Cytarabine
Used in paediatric AML protocols. Intrathecal cytarabine is used for CNS-directed therapy. Cerebellar toxicity less common in children at standard doses.
👴 Elderly
Anthracyclines
Higher risk of cardiotoxicity; consider liposomal doxorubicin or reduced-dose daunorubicin (45 mg/m²) in patients >60 years with comorbidities.
High-dose cytarabine
Cerebellar toxicity significantly higher in patients >60 years — use standard-dose cytarabine for consolidation, or consider non-intensive strategies.
Azacitidine ± venetoclax
Standard non-intensive approach for AML in patients >75 years or with significant comorbidities. PBS Authority Required.
🫘 Renal Impairment
Cytarabine
No dose adjustment for standard dose. Reduce dose of high-dose cytarabine if CrCl <60 mL/min.
Methotrexate
Renally cleared — requires dose modification, aggressive hydration, alkalinisation of urine, and leucovorin rescue. Contraindicated if CrCl <10 mL/min.
Fludarabine
Dose reduce 50% if CrCl 30–70 mL/min; avoid if CrCl <30 mL/min.
🫁 Hepatic Impairment
Vincristine
Hepatically metabolised; reduce dose 50% if bilirubin 1.5–3× ULN; avoid if bilirubin >3× ULN.
Anthracyclines
Hepatically cleared; reduce dose if bilirubin >1.5× ULN. Use idarubicin with caution.
🛡️ Immunocompromised
Fludarabine / purine analogues
Cause profound and prolonged CD4+ T-cell depletion (6–12 months post-treatment). Mandatory PJP and HSV/VZV prophylaxis. Irradiate all blood products.
Rituximab
Causes prolonged B-cell depletion and hypogammaglobulinaemia. Monitor immunoglobulin levels; IVIg replacement if recurrent severe infections.

Aboriginal and Torres Strait Islander Health Considerations

Aboriginal and Torres Strait Islander Health
Incidence & Mortality
Aboriginal and Torres Strait Islander peoples experience significantly higher incidence and mortality from haematological malignancies compared to non-Indigenous Australians. Lymphoma mortality rates are approximately 1.5–2 times higher, with later-stage presentation and reduced access to specialist haematology care contributing to poorer outcomes.
Geographic & Service Access
Many Aboriginal and Torres Strait Islander patients live in regional or remote areas distant from tertiary haematology centres. Treatment with intensive chemotherapy (7+3, HyperCVAD) requires prolonged hospital admission in metropolitan centres, creating significant family and community separation. Telehealth consultation for post-cycle monitoring is increasingly used.
Cultural Safety in Chemotherapy
Use Aboriginal Health Workers and Liaison Officers to support patient education about chemotherapy regimens, expected side effects, and the importance of completing prescribed cycles. Provide culturally appropriate written materials. Respect connection to country and community during hospitalisation.
Comorbidity Burden
Higher rates of renal impairment, diabetes, cardiovascular disease, and hepatitis B may complicate chemotherapy delivery and require dose modification. Screen for hepatitis B (HBsAg, anti-HBc, anti-HBs) before rituximab, as reactivation risk is significant.
Supportive Care Gaps
Access to supportive care services — including allied health, psychology, nutrition, and financial counselling — may be limited in remote communities. Coordinate with Aboriginal Community Controlled Health Organisations (ACCHOs) and use Closing the Gap PBS co-payment programs to reduce medication costs.
Clinical Trial Representation
Aboriginal and Torres Strait Islander peoples are underrepresented in haematological clinical trials. Active efforts to improve recruitment, ensure trial sites are culturally safe, and provide travel/accommodation support are underway through national cooperative group trials (ALLG, ANZCHOG).

📚 References

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