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Eosinophilia Syndromes

📋 Key Information Summary

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  • Definition: Peripheral eosinophilia is an absolute eosinophil count (AEC) ≥0.5 × 10⁹/L; moderate eosinophilia 1.5–5.0 × 10⁹/L; hypereosinophilia (HE) ≥1.5 × 10⁹/L persisting for ≥1 month or associated with tissue infiltration.
  • Classification: Eosinophilia is broadly divided into secondary (reactive) causes (~90%), primary (clonal/neoplastic) causes, and idiopathic — this distinction drives investigation and management.
  • Most common causes in Australia: Allergic/atopic disease, helminth infections (Strongyloides, hookworm in ATSI communities), drug reactions, and malignancy in older adults.
  • Hypereosinophilic syndrome (HES): Requires AEC ≥1.5 × 10⁹/L for ≥6 months with end-organ damage; FIP1L1-PDGFRA rearrangement must be tested in all patients as it responds to imatinib.
  • End-organ damage: Cardiac (Loeffler endocarditis — 50–60% of HES), pulmonary, neurological, dermatological, and gastrointestinal involvement determines prognosis.
  • Emergency presentations: Acute eosinophilic myocarditis or encephalitis require urgent high-dose corticosteroids (prednisolone 1 mg/kg/day or IV methylprednisolone 500–1000 mg).
  • First-line treatment: Prednisolone 0.5–1 mg/kg/day for symptomatic HES; imatinib 100 mg daily for FIP1L1-PDGFRA-positive HES (PBS Authority Required).
  • Investigation pathway: FBC with differential, reticulocyte count, B12, tryptase, FIP1L1-PDGFRA by FISH/PCR, bone marrow biopsy with cytogenetics, and stool OCP/serology for Strongyloides.
  • Tropical infections: Strongyloides hyperinfection can mimic HES; always exclude before initiating immunosuppression in patients from endemic regions including remote Northern Australia.
  • ATSI considerations: Higher prevalence of soil-transmitted helminths and Strongyloides; increased barriers to specialist referral in remote communities; consider empirical ivermectin where strong suspicion exists.
  • MBS availability: FBC/differential (MBS 65070), serum B12 and tryptase are generally available; FIP1L1-PDGFRA testing requires referral to specialised haematology/genetics laboratory.
  • Monitoring: Serial echocardiography and troponin for cardiac involvement; troponin elevation or reduced LVEF warrants urgent cardiology referral.

Introduction & Australian Epidemiology

Eosinophilia syndromes encompass a broad spectrum of disorders characterised by the accumulation of eosinophils in the peripheral blood and/or tissues. They range from mild, self-limiting reactive eosinophilia to life-threatening conditions with irreversible end-organ damage. The peripheral absolute eosinophil count (AEC) provides a severity grading framework: mild (0.5–1.5 × 10⁹/L), moderate (1.5–5.0 × 10⁹/L), and severe (≥5.0 × 10⁹/L). Hypereosinophilia is defined as AEC ≥1.5 × 10⁹/L on two occasions at least one month apart, or tissue hypereosinophilia with marked eosinophilic infiltration.

In Australia, eosinophilia is a relatively common incidental finding on full blood count, with mild eosinophilia present in 3–5% of general pathology samples. The aetiology varies significantly by geography and demographics. In temperate metropolitan areas, allergic and atopic disease dominate, whereas in tropical and remote northern Australia, helminth infections — particularly Strongyloides stercoralis and hookworm — remain important causes. Aboriginal and Torres Strait Islander communities bear a disproportionate burden of parasitic eosinophilia, with Strongyloides seroprevalence reported at 15–60% in some remote communities.

Hypereosinophilic syndromes are rare, with an estimated incidence of 0.36–6.3 per 100,000 person-years in international registries, and a male-to-female ratio of 9:1 for myeloproliferative HES variants. Australian haematology referral data suggest approximately 40–80 new HES diagnoses per year nationally. Early identification of the underlying aetiology is critical, as treatment differs markedly between reactive, clonal, and idiopathic subtypes.

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Safety alert — Strongyloides screening: Always screen for Strongyloides serology before initiating corticosteroids or immunosuppressive therapy in patients with unexplained eosinophilia. Immunosuppression can trigger fatal Strongyloides hyperinfection syndrome, particularly in patients from endemic regions (tropical Queensland, NT, Pacific Islands, Southeast Asia).
Eosinophilia Syndromes clinical infographic — pathophysiology, clinical clues, diagnosis, imaging, and management
Tap or click image to enlarge — Eosinophilia Syndromes: pathophysiology, clinical clues, diagnosis, imaging, and management.
Eosinophilia Syndromes infographic, full size

Causes & Classification

The 2012 Revised International Classification of eosinophilic disorders uses the terms hypereosinophilia (HE — blood or tissue criterion) and hypereosinophilic syndrome (HE + end-organ damage). Eosinophilia is stratified by aetiology into three major categories:

Category Subtype Key Examples
Secondary (Reactive) Allergic/Atopic Asthma, allergic rhinitis, atopic dermatitis, drug hypersensitivity (penicillins, NSAIDs, carbamazepine)
Infectious Helminths (Strongyloides, hookworm, Toxocara, Schistosoma), Strongyloides hyperinfection, visceral larva migrans
Neoplastic T-cell lymphoma (Sézary syndrome), Hodgkin lymphoma, solid tumours, mast cell neoplasia with eosinophilia
Primary (Clonal) Myeloproliferative FIP1L1-PDGFRA+ myeloid neoplasm, PDGFRB rearrangement, FGFR1 rearrangement (8p11 syndrome)
Lymphoproliferative Lymphocytic variant HES (clonal T-helper cells producing IL-5), chronic eosinophilic leukaemia NOS
Idiopathic Diagnosis of exclusion; all reactive and clonal causes excluded
Organ-restricted Eosinophilic oesophagitis, eosinophilic granulomatosis with polyangiitis (EGPA/Churg-Strauss), eosinophilic fasciitis

Stepwise Diagnostic Approach

A systematic approach to unexplained eosinophilia reduces the time to diagnosis and prevents inappropriate immunosuppression:

1
Confirm True Eosinophilia
Repeat FBC with manual differential to exclude pseudo-eosinophilia (toxic granulation, artefact). Ensure blood sample is fresh (eosinophil count drops with time).
2
Exclude Secondary Causes
Detailed allergy history (atopy, drug exposure); stool OCP ×3, Strongyloides serology, Toxocara serology; peripheral blood film for blast cells.
3
Screen for Clonal Disease
Serum B12, tryptase; FIP1L1-PDGFRA by FISH/PCR on peripheral blood; T-cell receptor gene rearrangement; flow cytometry for aberrant T-cell populations.
4
Assess End-Organ Damage
Troponin, echocardiography, pulmonary function tests, CT chest, dermatology review, neurological assessment. If HE present, bone marrow biopsy with cytogenetics.

Hypereosinophilic Syndrome

Hypereosinophilic syndrome (HES) is diagnosed when all three criteria are met: (1) AEC ≥1.5 × 10⁹/L on ≥2 occasions or tissue HE with marked eosinophilic infiltration; (2) exclusion of secondary/reactive causes; and (3) signs and symptoms of end-organ damage attributable to eosinophilia. HES is further sub-classified by the presence or absence of identifiable clonal markers.

Myeloproliferative HES (M-HES)

The FIP1L1-PDGFRA fusion gene results from an interstitial deletion on chromosome 4q12 (detected by FISH or RT-PCR). It encodes a constitutively active tyrosine kinase driving eosinophil proliferation. Features include male predominance, splenomegaly, elevated serum B12 (>1500 pmol/L), elevated tryptase (>20 µg/L), anaemia, and thrombocytopenia. Cardiac involvement occurs in 50–60% and is the leading cause of morbidity and mortality.

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Imatinib
Glivec® · Tyrosine kinase inhibitor
Adult dose (FIP1L1-PDGFRA+) 100 mg PO daily (lower than CML dose); titrate to 400 mg if inadequate response
Paediatric dose 260 mg/m² PO daily (max 400 mg); limited paediatric HES data
Route Oral
Duration Lifelong; molecular monitoring every 3–6 months
Renal adjustment eGFR 20–50: max 400 mg daily; eGFR <20: max 100 mg daily; not dialysable
Hepatic adjustment Mild–moderate impairment: use with caution; severe: avoid
PBS status 🔒 PBS Authority Required
Imatinib response: FIP1L1-PDGFRA+ HES has >95% haematological response rate to imatinib. Molecular remission (undetectable fusion transcript) is achievable and should be the treatment target.

Lymphocytic Variant HES (L-HES)

Clonal T-helper lymphocytes (often CD3⁻CD4⁺) produce IL-5 and other eosinophilopoietic cytokines. Distinguishing features: normal or mildly elevated B12/tryptase, absence of PDGFRA mutations, and elevated IL-5 or TARC/CCL17. There is a 10–15% risk of transformation to T-cell lymphoma over 10–15 years of follow-up. Management: corticosteroids first-line; mepolizumab (anti-IL-5 monoclonal antibody) or interferon-α as steroid-sparing agents.

Idiopathic HES

Diagnosed when all reactive, clonal, and lymphoproliferative causes have been excluded. Requires ongoing surveillance (6–12 monthly) for clonal evolution. Corticosteroids are first-line treatment. Hydroxyurea, interferon-α, and mepolizumab are second-line steroid-sparing options.

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Mepolizumab
Nucala® · Anti-IL-5 monoclonal antibody
Adult dose (HES) 300 mg SC every 4 weeks
Route Subcutaneous injection
Renal/hepatic adjustment Not established; use with caution in severe impairment
PBS status 🔒 PBS Authority Required (HES indication)
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Prednisolone
Solone® · Generic · Corticosteroid
Adult dose 0.5–1 mg/kg/day PO; taper over 4–8 weeks to lowest effective dose
Paediatric dose 1–2 mg/kg/day PO (max 60 mg); taper as guided by AEC
Route Oral
Renal/hepatic No dose adjustment; monitor fluid retention
PBS status ✔ PBS General Benefit

End-Organ Manifestations

End-organ damage in HES results from direct eosinophilic infiltration and release of cytotoxic granule proteins (major basic protein, eosinophil cationic protein, eosinophil peroxidase). Any organ can be involved, but cardiac, pulmonary, neurological, dermatological, and gastrointestinal manifestations are most clinically significant.

Mild
Dermatological
Eosinophilic cellulitis (Wells syndrome), pruritic papules and nodules, angioedema, urticaria. Present in 50–60% of HES patients.
Setting: Outpatient dermatology
Moderate
Pulmonary & GI
Chronic cough, wheeze, pulmonary infiltrates (may mimic pneumonia), eosinophilic colitis, abdominal pain, diarrhoea. PFTs may show obstructive or restrictive pattern.
Setting: Specialist referral; may require inpatient monitoring
Severe
Cardiac & Neurological
Cardiac: Loeffler endocarditis — endomyocardial fibrosis, restrictive cardiomyopathy, valvular regurgitation, mural thrombi. Leading cause of death in HES. Neuro: Peripheral neuropathy, cerebral thromboembolism from cardiac thrombi, encephalopathy.
Setting: Inpatient; cardiology/neurology referral; ICU if haemodynamically unstable

Cardiac Involvement — Loeffler Endocarditis

Cardiac disease is the most important prognostic determinant in HES. The pathophysiology progresses through three stages:

1
Acute Necrotic Stage
Eosinophilic myocarditis with necrosis. Elevated troponin, pericardial effusion, reduced LVEF. Responds to corticosteroids if caught early.
2
Thrombotic Stage
Mural thrombus formation on necrotic endocardium. Risk of systemic embolisation (stroke, limb ischaemia). Requires anticoagulation.
3
Fibrotic Stage
Endomyocardial fibrosis causing restrictive cardiomyopathy. Irreversible; may require valve replacement or cardiac transplantation.
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Acute eosinophilic myocarditis — medical emergency: Presents with acute heart failure, troponin rise, and reduced LVEF. Immediate high-dose IV methylprednisolone 500–1000 mg daily for 3 days, then oral prednisolone 1 mg/kg/day. Consult cardiology urgently. Anticoagulation if mural thrombus present. Consider inotropes/ICU if cardiogenic shock.

Pulmonary Involvement

Pulmonary eosinophilia may present as simple peripheral infiltrates (Löffler syndrome — often parasitic), chronic eosinophilic pneumonia (bilateral peripheral opacities, "photographic negative of pulmonary oedema"), or as part of EGPA. PFTs should include spirometry and DLCO. Bronchoalveolar lavage eosinophilia (>25% eosinophils) supports diagnosis when tissue sampling is not feasible.

Neurological Involvement

Peripheral neuropathy (mononeuritis multiplex or symmetric polyneuropathy) affects 5–15% of HES patients. Central nervous system involvement is usually thromboembolic (secondary to cardiac mural thrombi) rather than direct eosinophilic infiltration. Cerebral embolisation warrants cardiac MRI and consideration of long-term anticoagulation.

Dermatological Involvement

Cutaneous manifestations are often the presenting feature of HES. Eosinophilic cellulitis (Wells syndrome) presents with tender, oedematous plaques that may evolve through violaceous to granulomatous phases. Skin biopsy shows flame figures (collagen coated with degranulated eosinophil major basic protein). Treatment is with topical or systemic corticosteroids.

Investigations & Treatment

Investigations

Investigations should be tiered according to the severity and suspected aetiology. All patients with unexplained eosinophilia (AEC ≥1.0 × 10⁹/L) require a minimum baseline panel. Patients with AEC ≥1.5 × 10⁹/L or end-organ damage require the extended panel.

Essential
FBC with manual differential & peripheral blood film
MBS 65070 — universally available. Assess eosinophil morphology, blast cells, dysplastic features.
Essential
Stool OCP ×3 + Strongyloides serology
Exclude helminth infection before any immunosuppression. Strongyloides IgG ELISA sensitivity >90%.
Available
Serum B12 level
MBS 66837 — elevated B12 (>800 pmol/L) suggests myeloproliferative disease.
Available
Serum tryptase
Elevated (>20 µg/L) suggests myeloproliferative HES or systemic mastocytosis with eosinophilia. Available at major pathology providers.
Specialist
FIP1L1-PDGFRA by FISH and/or RT-PCR
Performed on peripheral blood or bone marrow. Referral to specialised haematology/genetics laboratory. Critical — imatinib-responsive.
Specialist
Bone marrow biopsy with cytogenetics and flow cytometry
Required for AEC ≥1.5 × 10⁹/L or suspected clonal disease. Assess cellularity, blast percentage, fibrosis, aberrant T-cell populations.
Available
Troponin & echocardiography
Screen for cardiac involvement. If abnormal → cardiac MRI with gadolinium, consider endomyocardial biopsy.
Available
T-cell receptor gene rearrangement & IL-5 levels
Assess for lymphocytic variant HES. IL-5 and TARC/CCL17 available at specialist immunology laboratories.

Treatment Algorithm

Treatment is determined by the underlying aetiology and presence of end-organ damage:

1
Treat the Underlying Cause
Helminth infection → ivermectin or albendazole. Drug reaction → withdraw offending agent. Allergic disease → standard allergy management. Neoplasm → directed oncology/haematology therapy.
2
FIP1L1-PDGFRA Positive
Imatinib 100 mg PO daily — first-line, highly effective. Monitor FIP1L1-PDGFRA transcript every 3–6 months. Lifelong therapy; molecular relapse occurs rapidly upon cessation.
3
HES Without FIP1L1-PDGFRA
Prednisolone 0.5–1 mg/kg/day (first-line). If steroid-dependent or refractory: mepolizumab 300 mg SC q4wk, hydroxyurea, or interferon-α.
4
Cardiac Complications
Acute myocarditis: IV methylprednisolone. Thromboembolism: anticoagulation (warfarin or DOAC). Restrictive cardiomyopathy: cardiology + heart failure management ± transplantation assessment.

Pharmacological Agents

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Hydroxyurea
Hydrea® · Antimetabolite
Adult dose 500 mg–1 g PO daily; titrate to AEC <1.0 × 10⁹/L
Renal adjustment eGFR <30: reduce dose by 50%; avoid in dialysis
Monitoring FBC fortnightly initially; watch for myelosuppression
PBS status ✔ PBS General Benefit
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Interferon alfa-2a
Roferon-A® · Cytokine modulator
Adult dose 3 million units SC 3 times weekly; titrate up to 9 MU weekly
Side effects Flu-like symptoms, depression, cytopenias — requires psychiatric screening
PBS status 🔒 PBS Authority Required
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Ivermectin
Stromectol® · Anthelmintic
Adult dose (Strongyloides) 200 µg/kg PO, repeat day 2 (standard); hyperinfection: daily for 5–7 days or until negative
Paediatric dose 200 µg/kg PO (children ≥15 kg)
PBS status ✔ PBS General Benefit

Monitoring Plan

Parameter Frequency Action Threshold
FBC with differential Weekly during active treatment; monthly when stable AEC >1.5 × 10⁹/L → escalate therapy
Troponin Every 3–6 months if prior cardiac involvement Any rise → urgent echo + cardiology referral
Echocardiography Baseline, 3 months, then annually New wall motion abnormality, EF decline, or valvular disease
FIP1L1-PDGFRA transcript Every 3–6 months on imatinib Rising transcript → assess compliance, consider dose escalation
T-cell markers + IL-5 Every 6–12 months for L-HES Clonal expansion → re-evaluate for lymphoma transformation

Special Populations

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Pregnancy
Prednisolone: Considered safe in pregnancy (Category A). Use lowest effective dose. Monitor for gestational diabetes.
Imatinib: Teratogenic (Category D) — contraindicated in first trimester. Consider dose reduction or temporary cessation with close monitoring; rechallenge after first trimester if FIP1L1-PDGFRA+ with aggressive disease. Multidisciplinary discussion essential.
Mepolizumab: Limited human data. Animal studies show no teratogenicity. Use only if benefit clearly outweighs risk.
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Paediatrics
HES is very rare in children. Reactive causes (parasites, allergy) are far more common. Exclude helminth infection before any immunosuppression.
Imatinib: Paediatric dose 260 mg/m² daily (max 400 mg). Limited data in paediatric HES; extrapolate from CML paediatric experience.
Mepolizumab is approved for severe eosinophilic asthma in children ≥6 years; HES use is off-label in paediatrics.
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Renal Impairment
Imatinib: eGFR 20–50: max 400 mg daily. eGFR <20: max 100 mg daily. Not significantly removed by haemodialysis.
Hydroxyurea: Reduce dose by 50% if eGFR <30; avoid in dialysis.
Corticosteroids: no dose adjustment but monitor for fluid retention and hyperglycaemia.
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Hepatic Impairment
Imatinib: Metabolised hepatically (CYP3A4). Mild–moderate impairment: use with caution. Severe impairment: avoid or use 25% dose.
Prednisolone: no dose adjustment but increased risk of fluid retention. Monitor LFTs.
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Immunocompromised
Patients on corticosteroids or imatinib are immunosuppressed. Ensure Pneumocystis jirovecii prophylaxis (co-trimoxazole) if prolonged prednisolone use.
Live vaccines contraindicated during immunosuppression. Ensure influenza and pneumococcal vaccination prior to commencing therapy.

Aboriginal and Torres Strait Islander Health

Aboriginal and Torres Strait Islander Health Considerations

Eosinophilia syndromes in Aboriginal and Torres Strait Islander populations are disproportionately driven by infectious aetiologies, particularly soil-transmitted helminths. Strongyloides stercoralis seroprevalence ranges from 15–60% in some remote NT and Queensland communities, compared to <1% in non-Indigenous metropolitan populations. Hookworm, Toxocara, and Schistosoma species (in communities with travel to endemic regions) should be considered.

Infectious aetiology prevalence
Soil-transmitted helminths are the most common cause of moderate-to-severe eosinophilia in remote ATSI communities. Always test Strongyloides serology and stool OCP before attributing eosinophilia to a non-infectious cause.
Remote access to specialist care
Bone marrow biopsy and FIP1L1-PDGFRA testing require metropolitan or major regional centre referral. Telehealth haematology consultation via the Royal Flying Doctor Service or jurisdictional telehealth programmes should be utilised early.
Empirical treatment
In remote settings where specialist referral is delayed and Strongyloides serology is positive or pending, empirical ivermectin 200 µg/kg PO × 2 days is appropriate. Do not start corticosteroids until Strongyloides is excluded or treated.
Pharmacovigilance
Imatinib requires molecular monitoring every 3–6 months (FIP1L1-PDGFRA transcript). Arrange sample transport to a reference laboratory. Where this is not feasible, clinical and FBC monitoring are acceptable interim measures.
Cultural safety
Engage Aboriginal Health Workers and Liaison Officers in discussion about invasive procedures (bone marrow biopsy). Provide culturally appropriate education materials in language where available. Respect family and community involvement in healthcare decisions.
AIHW data
Aboriginal and Torres Strait Islander Australians have higher rates of hospitalisation for parasitic disease. Community-wide Strongyloides screening and treatment programmes (as recommended by RHDAustralia) should be supported to reduce the burden of reactive eosinophilia.

📚 References

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  2. 2. Gotlib J. World Health Organization-defined eosinophilic disorders: 2022 update on diagnosis, risk stratification, and management. American Journal of Hematology. 2022;97(8):1089-1108.
  3. 3. Cools J, DeAngelo DJ, Gotlib J, et al. A tyrosine kinase created by fusion of the PDGFRA and FIP1L1 genes as a target of imatinib in idiopathic hypereosinophilic syndrome. New England Journal of Medicine. 2003;348(13):1201-1214.
  4. 4. Klion AD, Bochner BS, Gleich GJ, et al. Approaches to the treatment of hypereosinophilic syndromes: a workshop summary report. Journal of Allergy and Clinical Immunology. 2006;117(6):1292-1302.
  5. 5. Rothenberg ME, Klion AD, Roufosse FE, et al. Treatment of patients with the hypereosinophilic syndrome with mepolizumab. New England Journal of Medicine. 2008;358(12):1215-1228.
  6. 6. Helbig G, Hus M, Francuz T, et al. Characteristics and clinical outcome of patients with hypereosinophilic syndrome: a single-centre experience. Advances in Medical Sciences. 2013;58(2):285-291.
  7. 7. Currie BJ, McCarthy JS. Parasitic infections in tropical Australia. Medical Journal of Australia. 2010;192(8):432-437.
  8. 8. Shield JM, Vatui G, Betuela I. Strongyloides stercoralis infection in Australia: a neglected disease of Indigenous communities. Australian and New Zealand Journal of Public Health. 2005;29(1):71-76.
  9. 9. O'Connell EM, Nutman TB. Eosinophilia in infectious diseases. Immunology and Allergy Clinics of North America. 2015;35(3):493-522.
  10. 10. Bain BJ. Eosinophilia — idiopathic or secondary? Blood. 2021;137(22):3033-3034.
  11. 11. Roufosse F, Weller PF. Practical approach to the patient with hypereosinophilia. Journal of Allergy and Clinical Immunology. 2010;126(1):39-44.
  12. 12. RHDAustralia. Strongyloides stercoralis: Guidelines for the Diagnosis, Treatment and Prevention of Strongyloidiasis. Darwin: RHDAustralia; 2022.
  13. 13. Australian Institute of Health and Welfare (AIHW). Aboriginal and Torres Strait Islander Health Performance Framework 2020 Summary Report. Canberra: AIHW; 2020.
  14. 14. Schwartz LB, Metcalfe DD, Miller JS, et al. Tryptase levels as an indicator of mast-cell activation in systemic anaphylaxis and mastocytosis. New England Journal of Medicine. 1987;316(26):1622-1626.